Sapphire Wafers for Oil-Immersion Microscopy

university wafer substrates

Thin Sapphire for Oil Immersion Microscopy

A scientist contacted us for a quote:

"I notice there is 2' (50.8mm) wafer available on your website and I can go with that. We are using this wafer for oil-immersion microscopy so the thickness under 150um is necessary."

UniversityWafer, Inc. Quoted:

50.8mm 100um DSP c-plane off to M plane 0.2 deg wafers.

Price depends on on quantity.

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What Sapphire Wafers Are Used for Oil Immersion-Microscopy

Oil-immersion microscopy (OIM) is a technique used to study minute structural detail. It was invented by Konrad Zobitz in 1950. The goal of the microscope is to let the researcher see minute structural details that would otherwise be missed with a regular optical microscope. OIM uses a dilution of oil and a thin film fluorescent dye. The oil-dye combination is placed onto a slide, which is covered with a micron-sized layer of green material. The microscope magnifier directs a bright light through the slides to illuminate the area to be inspected.

 

 

The sapphire is used as an experimental control for the microscope. The sapphire can be viewed on the top, bottom, and front of the specimen. The sapphire may appear brown, green, blue, white, or black on the interior surface of the microscope slide. Because the inside of the slide is transparent to the eye, this technique provides high magnification viewing of minute internal structures.

The technique was used in the field of bacteriology when doctors could not examine organs like the liver, pancreas, or kidneys with conventional methods. They could not study physiology or anatomy, and they could not view cellular functions at the level of the organ because it was too small. When they used a SEM, or microscopic scope, they could look at these structures, but only under a microscope. One example of a good bacteriological instrument is the wellspray and the polished sapphire.

In addition to the above example, the sapphire can also be used in oil-immersion microscopy with a quartz tube. A sapphire-coated quartz tube has been heated to approximately 6000 degrees Fahrenheit. The sapphire-coated tube is then placed inside a plastic cup. A secondary bead-like structure sits on top of the quartz tube. Because the sapphire-coated tube is transparent to the eye, the oil-immersion microscope can clearly see the internal workings of the specimen.

The sample material is placed into the wellspray, which is covered by oil. The sample material, glassware, for example, is placed inside the wellspray. Then, the sample material is placed inside the SEM. Finally, the sample is placed inside the microscope. This procedure produces three clear images: one through the eyepiece lens, one through the SEM, and one through the objective lens.

The light from the source can be reflected on the specimen, or it can pass through the specimen and enter the objective lens. When the light passes through the specimen, the instrument can observe any fluorescence that may be present. Fluorescent materials sometimes demonstrate biological activity.

The technique was first described in 1938 by Carl Wilhelm Scheele. Later, the technique was patented inuble in soluble form by Wilhelm Roeland. The latest version of the fluorescent microscope is the Raman spectrometer, which is more sensitive than the former compound microscope. Some of the novel fluorescent microscope models have been developed and are now in use. One such model is the Fluorex enabled microscope, which can differentiate between normal cells and abnormal cells using only light from the fluorescent source.

Before the sample can be placed inside the instrument, some small droplets of oil need to be soaked for several minutes. After this stage, the sample can be placed inside the specimen. After the sample has been placed inside, the oil-immersion procedure can begin. The sample can be studied with the aid of the instrument for several hours before the sample is removed from the device for analysis.

Oil-immersion microscopy is a highly specialized type of microscope and must be used in an appropriate environment. In this type of microscopy, it is not advisable to use a sample slide covered in a glass cover for protection, since the glass cover blocks the fluorescent microscope light from entering the sample. This prevents the fluorescent signal from bouncing off the sample and hence reduces the intensity of the image. The sample should be placed on a microscope slide that has been previously coated with a gel or protein solution so that the sample will retain the fluorescent signal.

A specially designed microplate can also prevent the sample from scattering. However, this is not necessary if the slide is used in a normal microscope. Once the sample is inside the microscope, the oil droplets condense on the slide surface as the sample moves through the tube. This is called slowing of the sample. Oil droplets can be easily detected using a scanning electron microscope or a laser/ultraviolet lamp.

The microtube structure absorbs the oil droplets and allows them to be viewed easily with a highly sensitive carbon filter wheel. If the structure is non-aligned, the sample will display muddiness whereas an aligned structure produces a sharp image. Once the image is registered on the carbon plate, the sample is placed in a glass cylinder filled with water. Oil drops evaporate and the whole procedure is then repeated until the desired image is obtained.